The calaCLEAR high resolution microscope slide is the same size and shape as a conventional microscope slide and can be handled, labelled, racked and stained, by hand or by automated strainers, using existing standard laboratory equipment. calaCLEAR
microscope slides are formed of anodised aluminium (stiff, chemically resistant and non-corrosive) with a 35mm x 20mm access hole to allow tissue sections or cytological preparations to be applied onto the under-surface of a pre-applied, high quality borosilicate
Below is a diagram illustrating the two key differences between the calaCLEAR
high resolution microscope slide and a conventional microscope slide.
Firstly, for bright field microscopy, it can be seen that the light passing up through
the tissue section does not first pass through one millimetre of glass (as for the
conventional microscope slide) thereby avoiding unwanted light diffraction and
refractive dispersion. Secondly, the “mountant/sealant” (DPX or calaSEAL) is beneath
the tissue section rather than between the section and the coverslip. This eliminates
the refractive index discrepancy between the (coverslip) mountant and the coverslip
for light that has passed through the tissue section/cytological preparation thereby
significantly reducing both spherical aberration and chromatic aberration. In essence,
by removing the unnecessary optical interference inherent in the design of the
conventional microscope slide, the calaCLEAR slide improves the microscopic
Because of the reduction in optical aberration, the microscopical image not only
appears sharper but also thinner and flatter than with a conventional microscope slide.
This translates to a decreased depth of focus and, when used for digital microscopy, this image flatness can significantly reduce the number of z-axis scans required and, consequently, the total scanning time for whole slide imaging (WSI). This has important efficiencies, not only in decreased scanning time but also in reducing the size of the resultant digital image files – a major cost benefit for archiving digital images.
For a more detailed explanation of the importance of eliminating the coverslip mountant above the tissue section, read more by following this link.
Beyond the optical advantages, use of the calaCLEAR microscope slides has a number of additional benefits over conventional glass slides:
1) The anodised aluminium finish is a perfect substrate for laser marking. Compared to the ink option most commonly employed with conventional glass slides (either powder-coated painted labelling areas or with expensive applied chemically-resistant labels), the laser marking of the calaCLEAR slide is fast, truly permanent/resistant to all chemicals and with no costly ink consumables. The crisp laser marking means that font size can be reduced and more text can be applied to the slides per unit area. Small, modern YAG lasers are comparable in price to microscope slide ink printers but with significantly lower maintenance costs. Please contact us if you wish to explore the laser-marking option.
2) Because there is no application of a coverslip over the tissue or cytological preparation, there are no annoying trapped bubbles - ever!
3) The calaCLEAR slides are approximately 50% lighter helping bring down costs of archival storage.
Copyright © Calamat Ltd. Reg.No. 07614030. All Rights Reserved. calaCLEAR microscope slide – patent pending, UK patent number 1403822.8. PCT/GB2015/050616. international patent application number WO 2015/132583